pCI Cas9 H2B-RFP
(Plasmid #92397)

• Purpose: CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES
• Depositing Lab: Tatjana Sauka-Spengler
• Publication: Williams et al Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333.

Backbone

• Vector backbone: pCI IRES H2B RFP
• Backbone size w/o insert (bp): 6714
• Total vector size (bp): 10815
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Insert Size (bp): 4101
• Promoter: CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCTCATCGATAAGCTTGATGCCACcatggcccca
• 3′ sequencing primer: CCGGGCTGCAGGAATTCGATctatcattactttttcttttttgcctggcc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cas9 was cloned into the EcoRV site of pCI IRES H2B RFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.