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Addgene

pAAV-M13-TEV-C-P2A-TdTomato
(Plasmid #92391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSyc 171 P2A vector
  • Total vector size (bp) 6446
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M13-TEV-C-P2A-TdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    2025
  • Promoter human synapsin
  • Tag / Fusion Protein
    • TdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-M13-TEV-C-P2A-TdTomato was a gift from Hyungbae Kwon (Addgene plasmid # 92391 ; http://n2t.net/addgene:92391 ; RRID:Addgene_92391)

  • For your References section:

    A calcium- and light-gated switch to induce gene expression in activated neurons. Lee D, Hyun JH, Jung K, Hannan P, Kwon HB. Nat Biotechnol. 2017 Jun 26. doi: 10.1038/nbt.3902. 10.1038/nbt.3902 PubMed 28650460
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