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pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
(Plasmid #92390)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSyc 171 P2A vector
  • Total vector size (bp) 6679
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
  • Species
    Synthetic
  • Insert Size (bp)
    2628
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer CMVf
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA was a gift from Hyungbae Kwon (Addgene plasmid # 92390 ; http://n2t.net/addgene:92390 ; RRID:Addgene_92390)

  • For your References section:

    A calcium- and light-gated switch to induce gene expression in activated neurons. Lee D, Hyun JH, Jung K, Hannan P, Kwon HB. Nat Biotechnol. 2017 Jun 26. doi: 10.1038/nbt.3902. 10.1038/nbt.3902 PubMed 28650460
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