pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
(Plasmid #92388)

• Purpose: Cell death reporter
• Depositing Lab: Nicholas Cole
• Publication: Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321. doi: 10.3389/fncel.2015.00321. eCollection 2015.

Backbone

• Vector backbone: pTol2pA2
• Backbone manufacturer: Tol2kit
• Backbone size w/o insert (bp): 5800
• Modifications to backbone: cryaa:RFP inserted
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ANNEXINV
• Alt name: ANXA5
• Alt name: annexin A5
• Species: H. sapiens (human)
• Insert Size (bp): 5000
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
• Promoter: ubb
• Tag / Fusion Protein:
  • mVenus (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pTol2pA2-ubb_secHsa.ANNEXINV-mVenus, cryaa_RFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry