Focused Ras Synthetic Lethal Human CRISPR Knockout Library
(Pooled Library #92352)

• Purpose:

  The Sabatini/Lander CRISPR pooled library is a focused gRNA library that targets synthetic lethal candidate and control genes with oncogenic Ras.

• Vector Backbone:

  lentiCRISPR v1 (Plasmid #49535) - expresses Cas9

  Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages users to use the updated lentiCRISPR v2 (Plasmid #52961), which can be requested separately.

• Depositing Labs: David Sabatini, Eric Lander
• Publication: Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013.

Library Details

• Species: Human
• Genes targeted: 132
• gRNAs: 6,661
• Controls: 499 intergenic
• Lentiviral Generation: 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼10 µL
• Concentration: 10 ng/µL

Resource Information

• Protocols:
  • Library Transformation Protocol (DOCX, 15 KB)
  • Screening Protocol (DOCX, 8.4 KB)
  • PCR protocol for Illumina sequencing preparation (DOCX, 15 KB)
• Depositor Data:
  • List of gRNA sequences (XLSX, 349 KB)
  • Sequencing data (initial NGS counts) (TXT, 123 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).

Figure 1: Distribution of sgRNA counts in the initial cell population.