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PurposeThe vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep23-phage
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry and split EGFP
- Promoter pTet
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer none specified (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tet-on circRNA Expression Vector was a gift from Ling-Ling Chen (Addgene plasmid # 92351 ; http://n2t.net/addgene:92351 ; RRID:Addgene_92351) -
For your References section:
The Biogenesis of Nascent Circular RNAs. Zhang Y, Xue W, Li X, Zhang J, Chen S, Zhang JL, Yang L, Chen LL. Cell Rep. 2016 Apr 19;15(3):611-24. doi: 10.1016/j.celrep.2016.03.058. Epub 2016 Apr 7. 10.1016/j.celrep.2016.03.058 PubMed 27068474
