Skip to main content
Addgene

pCDNA3-SPA mimic WT
(Plasmid #92350)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCDNA3
  • Backbone size w/o insert (bp) 5407
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4685
  • Entrez Gene
    SNRPN (a.k.a. HCERN3, PWCR, RT-LI, SM-D, SMN, SNRNP-N, SNURF-SNRPN, sm-N)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer TCTATAGTGTCACCTAAAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-SPA mimic WT was a gift from Ling-Ling Chen (Addgene plasmid # 92350 ; http://n2t.net/addgene:92350 ; RRID:Addgene_92350)

  • For your References section:

    Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins. Wu H, Yin QF, Luo Z, Yao RW, Zheng CC, Zhang J, Xiang JF, Yang L, Chen LL. Mol Cell. 2016 Nov 3;64(3):534-548. doi: 10.1016/j.molcel.2016.10.007. Epub 2016 Oct 27. 10.1016/j.molcel.2016.10.007 PubMed 27871485
Related items: