pEGFP-IRAlus-Lin28
(Plasmid
#92347)
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PurposePlasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-c1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLin28 gene derived IR Alu element containing 3'UTR
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SpeciesH. sapiens (human)
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Entrez GeneLIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-IRAlus-Lin28 was a gift from Gordon Carmichael & Ling-Ling Chen (Addgene plasmid # 92347 ; http://n2t.net/addgene:92347 ; RRID:Addgene_92347) -
For your References section:
Alu element-mediated gene silencing. Chen LL, DeCerbo JN, Carmichael GG. EMBO J. 2008 Jun 18;27(12):1694-705. doi: 10.1038/emboj.2008.94. Epub 2008 May 22. 10.1038/emboj.2008.94 PubMed 18497743
