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Addgene

pEGFP-Alu-Lin28
(Plasmid #92346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-c1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lin28 gene derived Alu element containing 3'UTR
  • Species
    H. sapiens (human)
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-Alu-Lin28 was a gift from Gordon Carmichael & Ling-Ling Chen (Addgene plasmid # 92346 ; http://n2t.net/addgene:92346 ; RRID:Addgene_92346)

  • For your References section:

    Alu element-mediated gene silencing. Chen LL, DeCerbo JN, Carmichael GG. EMBO J. 2008 Jun 18;27(12):1694-705. doi: 10.1038/emboj.2008.94. Epub 2008 May 22. 10.1038/emboj.2008.94 PubMed 18497743
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