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Addgene

pHdzCas9-KRAB
(Plasmid #92341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHdzCas9-KRAB
  • Backbone manufacturer
    Addgene
  • Backbone size (bp) 11169
  • Vector type
    Plant Expression, CRISPR ; Plant expression
  • Promoter CaMV35S
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer ATGGATTACAAGGACCACGACGGGGA
  • 3′ sequencing primer TACGATGTCCCTGACTACGCCTCATGA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHdzCas9-KRAB was a gift from I-Son Ng (Addgene plasmid # 92341 ; http://n2t.net/addgene:92341 ; RRID:Addgene_92341)

  • For your References section:

    CRISPRi mediated phosphoenolpyruvate carboxylase regulation to enhance the production of lipid in Chlamydomonas reinhardtii. Kao PH, Ng IS. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30619-3. doi: 10.1016/j.biortech.2017.04.111. 10.1016/j.biortech.2017.04.111 PubMed 28501380
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