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Addgene

pPickles-2.31
(Plasmid #92336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha is also a suitable growth strain.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pickles-2.31
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2101
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPickles-2.31 was a gift from Yusuke Ohba (Addgene plasmid # 92336 ; http://n2t.net/addgene:92336 ; RRID:Addgene_92336)

  • For your References section:

    A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, Ohba Y. Clin Cancer Res. 2010 Aug 1;16(15):3964-75. doi: 10.1158/1078-0432.CCR-10-0548. 10.1158/1078-0432.CCR-10-0548 PubMed 20670950
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