pJL006
(Plasmid #92329)

• Purpose: Express mEos3.2 tagged FtsZD212A mutant in E coli
• Depositing Lab: Jie Xiao
• Publication: Lyu et al Biopolymers. 2016 Oct;105(10):725-34. doi: 10.1002/bip.22895.

Backbone

• Vector backbone: pCA24N
• Backbone manufacturer: NBRP-E.coli at NIG
• Backbone size w/o insert (bp): 4500
• Modifications to backbone: replaced N-terminal 6xHis with novel SpeI site, results in 6bp reduced expression
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For fluorescence studies, grow at RT or 30C. Note the depositor uses BW25113 for molecular/imaging assays, as this strain provided a more “wild-type” background.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FtsZ
• Species: E. coli
• Insert Size (bp): 1149
• Mutation: D212A
• Promoter: T5-lac
• Tag / Fusion Protein:
  • mEos3.2 (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ctttcgtcttcacctcgagaaatc
• 3′ sequencing primer: gctaattaagcttggctgcaggt

Resource Information

• Supplemental Documents:
  • pJL006.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry