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pCGN-HCF-1-N450-1011
(Plasmid #92323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 5038
  • Total vector size (bp) 6772
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCF-1
  • Alt name
    HCFC1
  • Alt name
    host cell factor C1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1734
  • Mutation
    AA 450-1011
  • GenBank ID
    NM_005334.2 NP_005325.2
  • Entrez Gene
    HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (overlapping dam methylation) (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_for
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGN-HCF-1-N450-1011 was a gift from Winship Herr (Addgene plasmid # 92323 ; http://n2t.net/addgene:92323 ; RRID:Addgene_92323)

  • For your References section:

    VP16 targets an amino-terminal domain of HCF involved in cell cycle progression. Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W. Mol Cell Biol. 1997 Oct;17(10):6139-46. PubMed 9315674
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