pCGN-HCF-1-N1011Δ382-450
(Plasmid #92322)

• Purpose: N-term of HCF-1 protein without amino acids 382-450
• Depositing Lab: Winship Herr
• Publication: Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 5038
• Total vector size (bp): 7909
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HCF-1
• Alt name: HCFC1
• Alt name: host cell factor C1
• Species: H. sapiens (human)
• Insert Size (bp): 2871
• Mutation: AA 2-1011 with internal deletion Δ382-450
• GenBank ID: NM_005334.2 NP_005325.2
• Entrez Gene: HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (overlapping dam methylation) (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry