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PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonesynthetic
- Backbone size w/o insert (bp) 5238
- Total vector size (bp) 7446
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Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAncestral AAV Capsid Anc80L65AAP
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Alt nameANC80L65AAP
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SpeciesSynthetic
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Insert Size (bp)2208
- Promoter Rep2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAnc80L65AAP was a gift from Luk Vandenberghe (Addgene plasmid # 92307 ; http://n2t.net/addgene:92307 ; RRID:Addgene_92307) -
For your References section:
A synthetic AAV vector enables safe and efficient gene transfer to the mammalian inner ear. Landegger LD, Pan B, Askew C, Wassmer SJ, Gluck SD, Galvin A, Taylor R, Forge A, Stankovic KM, Holt JR, Vandenberghe LH. Nat Biotechnol. 2017 Mar;35(3):280-284. doi: 10.1038/nbt.3781. Epub 2017 Feb 6. 10.1038/nbt.3781 PubMed 28165475