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pY130 (pcDNA3.1-U6-huBsCpf1)
(Plasmid #92306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 10000
  • Modifications to backbone
    Insertion of U6::direct repeat BsmBI cloning cassette.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    huBsCpf1
  • Species
    Butyrivibrio sp. NC3005
  • Insert Size (bp)
    3618
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS-3xHA (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY130 (pcDNA3.1-U6-huBsCpf1) was a gift from Feng Zhang (Addgene plasmid # 92306 ; http://n2t.net/addgene:92306 ; RRID:Addgene_92306)
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