pY130 (pcDNA3.1-U6-huBsCpf1)
(Plasmid
#92306)
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PurposeExpression of humanized BsCpf1 and crRNA
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 10000
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Modifications to backboneInsertion of U6::direct repeat BsmBI cloning cassette.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuBsCpf1
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SpeciesButyrivibrio sp. NC3005
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Insert Size (bp)3618
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS-3xHA (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCloned by GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pY130 (pcDNA3.1-U6-huBsCpf1) was a gift from Feng Zhang (Addgene plasmid # 92306 ; http://n2t.net/addgene:92306 ; RRID:Addgene_92306)
