pY124 (pcDNA3.1-huBsCpf1)
(Plasmid #92300)

• Purpose: Expression of humanized BsCpf1
• Depositing Lab: Feng Zhang
• Publication: Zhang lab Cpf1 plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 10000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: huBsCpf1
• Species: Butyrivibrio sp. NC3005
• Insert Size (bp): 3618
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS-3xHA (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pcDNA3-huBsCpf1.gbk
• A portion of this plasmid was derived from a plasmid made by: Cloned by GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry