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Addgene

pZac2.1 hSynapsin1 FLEX NAPA-N SV40
(Plasmid #92283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZac2.1
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuron-astrocyte proximity assay - Neuronal
  • Alt name
    NAPA-N
  • Species
    Synthetic
  • Insert Size (bp)
    1653
  • Promoter hSynapsin1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GACCATCTGCGCTGCGGCG
  • 3′ sequencing primer GTGGTTTGTCCAAACTCATCAAT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZac2.1 hSynapsin1 FLEX NAPA-N SV40 was a gift from Baljit Khakh (Addgene plasmid # 92283 ; http://n2t.net/addgene:92283 ; RRID:Addgene_92283)

  • For your References section:

    An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance Scales. Octeau JC, Chai H, Jiang R, Bonanno SL, Martin KC, Khakh BS. Neuron. 2018 Apr 4;98(1):49-66.e9. doi: 10.1016/j.neuron.2018.03.003. 10.1016/j.neuron.2018.03.003 PubMed 29621490
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