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Addgene

pMCh-695
(Plasmid #92263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC5.1-NHA
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 9619
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dGW182 PAM2 mut
  • Alt name
    Gawky
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4164
  • Mutation
    The following point mutations were introduced: EF960AA; WK967AA; T982A.
  • GenBank ID
    NM_166783.2
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
  • Promoter Ac5
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACA CAA AGC CGC TCC ATC AG
  • 3′ sequencing primer CCT TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing finds two additional amino acid residue substitutions (V704A and N799S). The effects of these additional residue changes on protein function are not known.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-695 was a gift from Marina Chekulaeva & Witold Filipowicz (Addgene plasmid # 92263 ; http://n2t.net/addgene:92263 ; RRID:Addgene_92263)

  • For your References section:

    miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W. Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. 10.1038/nsmb.2166 PubMed 21984184
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