pMCh-56
(Plasmid #92260)

• Purpose: Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells
• Depositing Labs: Marina Chekulaeva, Roy Parker
• Publication: Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.1261/rna.1364909. Epub 2009 Mar 20.

Backbone

• Vector backbone: pAC5.1-HA
• Backbone size w/o insert (bp): 5361
• Total vector size (bp): 9525
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dGW182
• Alt name: Gawky
• Species: D. melanogaster (fly)
• Insert Size (bp): 4164
• GenBank ID: NM_166783.2
• Entrez Gene: gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
• Promoter: Ac5
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ACA CAA AGC CGC TCC ATC AG
• 3′ sequencing primer: CCT TAG AAG GCA CAG TCG AGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing finds two additional amino acid residue substitutions (V704A and N799S). The effects of these additional residue changes on protein function are not known.