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Addgene

pMCh-660
(Plasmid #92259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCiNeo-NHA
  • Backbone size w/o insert (bp) 5557
  • Total vector size (bp) 6551
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNRC6C CED
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    969
  • Mutation
    C-terminal effector domain (CED): aa 1369-1690 with the following point mutations: EF1388AA; W1445A; W1487A;W1494A; W1504A; W1605A; W1648A; W1659A
  • GenBank ID
    NM_001142640.1
  • Entrez Gene
    TNRC6C
  • Promoter CMV
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CACAGGTGTCCACTCCCAGTTC
  • 3′ sequencing primer ACTGCATTCTAGTTGTGGTTTGTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-660 was a gift from Marina Chekulaeva & Witold Filipowicz (Addgene plasmid # 92259 ; http://n2t.net/addgene:92259 ; RRID:Addgene_92259)

  • For your References section:

    miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W. Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. 10.1038/nsmb.2166 PubMed 21984184
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