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pJExpress414_GmPOPB-R663K
(Plasmid #92239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    DNA 2.0
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6229
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prolyl oligopeptidase B from Galerina marginata-R663K mutant
  • Alt name
    GmPOPB-R663K
  • Species
    Galerina marginata
  • Insert Size (bp)
    2193
  • Mutation
    Arginine 663 to Lysine
  • GenBank ID
    JN827314.2
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x-Histag, TEV cleavage site (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    DNA 2.0

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid to appear in incoming publication from our group - to be updated

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJExpress414_GmPOPB-R663K was a gift from James Naismith (Addgene plasmid # 92239 ; http://n2t.net/addgene:92239 ; RRID:Addgene_92239)

  • For your References section:

    Kinetic Landscape of a Peptide Bond-Forming Prolyl Oligopeptidase. Czekster CM, Naismith JH. Biochemistry. 2017 Apr 18;56(15):2086-2095. doi: 10.1021/acs.biochem.7b00012. Epub 2017 Apr 7. 10.1021/acs.biochem.7b00012 PubMed 28332820
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