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Addgene

CTL2
(Plasmid #92212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6222
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTL2 of PIEZO1
  • Alt name
    C Terminal Loop 2 of PIEZO1
  • Alt name
    PIEZO1
  • Species
    C. elegans (nematode)
  • GenBank ID
    392900032
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 6x Histidine Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promotoer
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CTL2 was a gift from Douglas Rees (Addgene plasmid # 92212 ; http://n2t.net/addgene:92212 ; RRID:Addgene_92212)

  • For your References section:

    The structure of a conserved piezo channel domain reveals a topologically distinct beta sandwich fold. Kamajaya A, Kaiser JT, Lee J, Reid M, Rees DC. Structure. 2014 Oct 7;22(10):1520-7. doi: 10.1016/j.str.2014.08.009. Epub 2014 Sep 18. 10.1016/j.str.2014.08.009 PubMed 25242456
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