MolBC
(Plasmid #92210)

• Purpose: Expression of H. influenzae MolBC (ABC Transporter) in E. coli
• Depositing Lab: Douglas Rees
• Publication: Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Epub 2006 Dec 7.

Backbone

• Vector backbone: pET21b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6763
• Modifications to backbone: Added the entire expression section of pET19b + MolC into pET21b(+) vector
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: MolC
• Alt name: H. influenzae ABC Transporter
• Alt name: HI1471
• Entrez Gene: HI1471 (a.k.a. HI1471)
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 8x Histidine Tag (N terminal on insert)
  • Enterokinase Cleavage Site between Histidine Tag and MolB (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: gccgttgagcaccgccgccgcaaggaatgg
• 3′ sequencing primer: cacgatatccaatacggaata

Gene/Insert 2

• Gene/Insert name: MolB
• Alt name: H. influenzae ABC Transporter
• Alt name: HI1470
• Entrez Gene: HI1470 (a.k.a. HI1470)
• Promoter: T7 Promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: tggttgatggggagttttgc
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • wi-h-influenzae-molc-hi1471molb-hi1470.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry