pBABE-α-Syn-nYFP
(Plasmid
#92203)
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PurposeRetroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE
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Backbone manufacturerZhu Songyang Lab (Baylor College of Medicine)
- Backbone size w/o insert (bp) 7247
- Total vector size (bp) 7666
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNCA
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SpeciesH. sapiens (human)
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Insert Size (bp)419
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
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Tag
/ Fusion Protein
- YFP(1-155) (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-α-Syn-nYFP was a gift from Huda Zoghbi (Addgene plasmid # 92203 ; http://n2t.net/addgene:92203 ; RRID:Addgene_92203) -
For your References section:
TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau. Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L, Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY. Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809. 10.7554/eLife.19809 PubMed 27779468