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pKH3-TRIM28-C65A/C68A
(Plasmid #92199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKH3
  • Backbone manufacturer
    Ian Macara; Fanxiu Zhu
  • Backbone size w/o insert (bp) 4807
  • Total vector size (bp) 7260
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tif1b
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2520
  • Mutation
    Changed Cysteines 65 and 68 to Alanines.
  • Entrez Gene
    TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH3-TRIM28-C65A/C68A was a gift from Huda Zoghbi (Addgene plasmid # 92199 ; http://n2t.net/addgene:92199 ; RRID:Addgene_92199)

  • For your References section:

    TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau. Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L, Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY. Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809. 10.7554/eLife.19809 PubMed 27779468
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