pLenti-DsRed_IRES_EGFP
(Plasmid #92194)

• Purpose: Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen
• Depositing Lab: Huda Zoghbi
• Publication: Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.

Backbone

• Vector backbone: pLenti-GPS-GAW-IRES
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 9942
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DsRed-IRES-EGFP
• Species: Synthetic
• Insert Size (bp): 2000

Resource Information

• Supplemental Documents:
  • pLenti-DsRed-IRES-GFP.gb
• A portion of this plasmid was derived from a plasmid made by: Modified from the pLenti-GPS-GAW-IRES vector obtained by Thomas F. Westbrook lab (Baylor College of Medicine)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry