Skip to main content
Addgene

pDIS3
(Plasmid #92173)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS314
  • Total vector size (bp) 6599
  • Vector type
    Yeast Expression ; GenBank: U03440.1
  • Selectable markers
    NAT1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NAT1
  • Species
    Candida albicans

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sacI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer GTGCCGTAAAGCACTAAATCGG
  • 3′ sequencing primer GTTACCTCACTCATTAGGCACCCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDIS3 was a gift from Judith Berman (Addgene plasmid # 92173 ; http://n2t.net/addgene:92173 ; RRID:Addgene_92173)

  • For your References section:

    Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans. Gerami-Nejad M, Zacchi LF, McClellan M, Matter K, Berman J. Microbiology. 2013 Mar;159(Pt 3):565-79. doi: 10.1099/mic.0.064097-0. Epub 2013 Jan 10. 10.1099/mic.0.064097-0 PubMed 23306673
Related items:
Commonly requested with: