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iRFP-PAmCherry1-PH_PLCd1
(Plasmid #92167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92167 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iRFP-PAmCherry1-PH_PLCd1
  • Insert Size (bp)
    2000
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP-PAmCherry1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer GTCGCGGCCGCTTACTCCTTCAGGAAGTTCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iRFP-PAmCherry1-PH_PLCd1 was a gift from Xuelin Lou (Addgene plasmid # 92167 ; http://n2t.net/addgene:92167 ; RRID:Addgene_92167)

  • For your References section:

    Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane. Ji C, Zhang Y, Xu P, Xu T, Lou X. J Biol Chem. 2015 Nov 6;290(45):26978-93. doi: 10.1074/jbc.M115.663013. Epub 2015 Sep 22. 10.1074/jbc.M115.663013 PubMed 26396197
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