pEHIShqprt
(Plasmid #92166)

• Purpose: Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
• Depositing Labs: Huanting Liu, James Naismith
• Publication: Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. Epub 2007 Aug 24.

Backbone

• Vector backbone: pEHISTEV
• Backbone manufacturer: BSRC, St Andrews
• Backbone size w/o insert (bp): 5364
• Total vector size (bp): 6247
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Protein expression in (DE3) strains with IPTG induction
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Quinolinate Phosphoribosyltransferase
• Alt name: hQPRTase
• Species: H. sapiens (human)
• Insert Size (bp): 894
• Mutation: changed Asp 2 to Asn to create BspHI site
• GenBank ID: BC018910.2
• Entrez Gene: QPRT (a.k.a. HEL-S-90n, QPRTase)
• Promoter: T7
• Tag / Fusion Protein:
  • TEV protease -cleavable N-terminal 6His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspHI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Originally cloned

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

none