PB-CAG-Haunt-EF1a-DsRed
(Plasmid
#92162)
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PurposeHaunt in trans overexpression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaunt
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SpeciesM. musculus (mouse)
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Insert Size (bp)3562
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Entrez GeneHalr1 (a.k.a. Gm15055, Haunt, Linchoxa1, linc-Evx1, linc-Hoxa1, linc1547)
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-CAG-Haunt-EF1a-DsRed was a gift from Xiaohua Shen (Addgene plasmid # 92162 ; http://n2t.net/addgene:92162 ; RRID:Addgene_92162) -
For your References section:
Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation. Yin Y, Yan P, Lu J, Song G, Zhu Y, Li Z, Zhao Y, Shen B, Huang X, Zhu H, Orkin SH, Shen X. Cell Stem Cell. 2015 May 7;16(5):504-16. doi: 10.1016/j.stem.2015.03.007. Epub 2015 Apr 16. 10.1016/j.stem.2015.03.007 PubMed 25891907