pLX_TRC208-SYNJ2BP-V5
(Plasmid #92149)

• Purpose: Expresses V5-tagged human SYNJ2BP in mammalian cells.
• Depositing Lab: Alice Ting
• Publication: Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.

Backbone

• Vector backbone: pLX_TRC208
• Backbone size w/o insert (bp): 8227
• Total vector size (bp): 8734
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SYNJ2BP-V5
• Alt name: OMP25-V5
• Species: H. sapiens (human)
• Insert Size (bp): 507
• Mutation: *(see below)
• Entrez Gene: SYNJ2BP (a.k.a. ARIP2, OMP25)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: CMVf

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original SYNJ2BP-V5 was obtained from the Broad Institute of MIT and Harvard.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The SYNJ2BP gene was modified to be resistant to the CRISPR guide RNAs used in Hung et al., eLife 2017.