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Addgene

pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 92142.

Expected result:

NotI-HF: 1 band at 9 kb
PvuI-HF: 2 bands at 8 and 1 kb