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Addgene

pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting with the following enzymes: EcoRI-HF, KpnI-HF, and XhoI.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting with the following enzymes: EcoRV-HF/ HindIII-HF.

We observe the expected bands for this digest.