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Addgene

pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpAFrt-PGK-EM7-PuroR-bpA-Frt Tigre targeting with the following enzymes: BamHI and MluI-HF.
Expected Results: 8122 bp and 2900 bp.
We observed the expected results.