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Addgene

pDHt/sk-PE
(Plasmid #92126)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDHt/sk
  • Vector type
    CRISPR ; Trichoderma reesei expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-eGFP
  • Promoter Ppdc
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDHt/sk-PE was a gift from Zhihua Zhou (Addgene plasmid # 92126 ; http://n2t.net/addgene:92126 ; RRID:Addgene_92126)

  • For your References section:

    Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Liu R, Chen L, Jiang Y, Zhou Z, Zou G. Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015. 10.1038/celldisc.2015.7 PubMed 27462408
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