YSBE 628 : pHYG_mCherry
(Plasmid
#92092)
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PurposePlasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTOP-V2
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Backbone manufacturerEnzynomics, Korea
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Vector typetagging proteins in Cryptococcus neoforman
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's sequencing results found K128R and Q193P mutations in mCherry. These mutations are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YSBE 628 : pHYG_mCherry was a gift from Yong-Sun Bahn (Addgene plasmid # 92092 ; http://n2t.net/addgene:92092 ; RRID:Addgene_92092) -
For your References section:
Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans. So YS, Yang DH, Jung KW, Huh WK, Bahn YS. J Microbiol Biotechnol. 2017 Feb 28;27(2):357-364. doi: 10.4014/jmb.1609.09036. 10.4014/jmb.1609.09036 PubMed 27780958
