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Addgene

YSBE 558 : pNAT_4xFLAG
(Plasmid #92086)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92086 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTOP-V2
  • Backbone manufacturer
    Enzynomics, Korea
  • Vector type
    tagging proteins in Cryptococcus neoforman
  • Selectable markers
    Nourseothricin (NAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4xFlag

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YSBE 558 : pNAT_4xFLAG was a gift from Yong-Sun Bahn (Addgene plasmid # 92086 ; http://n2t.net/addgene:92086 ; RRID:Addgene_92086)

  • For your References section:

    Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans. So YS, Yang DH, Jung KW, Huh WK, Bahn YS. J Microbiol Biotechnol. 2017 Feb 28;27(2):357-364. doi: 10.4014/jmb.1609.09036. 10.4014/jmb.1609.09036 PubMed 27780958
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