piggyBac CMV-GFP-FRT
(Plasmid #92078)

• Purpose: production of transgenic animal and recombination using flipase
• Depositing Lab: Jae Yong Han
• Publication: Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.1096/fj.15-274712. Epub 2015 Oct 6.

Backbone

• Vector backbone: pCyL50
• Backbone manufacturer: Sanger institute
• Backbone size w/o insert (bp): 3050
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV-eGFP and TK-Neo between FRT3 and FRT
• Insert Size (bp): 4000
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T3, M13R
• 3′ sequencing primer: M13F(-20)

Resource Information

• Supplemental Documents:
  • PB_CMV_EP_FRT (5-3) Addgene-2.gb
• A portion of this plasmid was derived from a plasmid made by: Sanger institute (http://www.sanger.ac.uk/ technology/clonerequests)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry