pSFVCs-lacZ
(Plasmid #92076)

• Purpose: This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.
• Depositing Lab: Henrik Garoff
• Publication: Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):1127-31.

Backbone

• Vector backbone: pGEM1
• Vector type: C-lacZ expression plasmid, for run-off transcription and RNA transfection of mammalian cells

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: C-lacZ

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Semliki forest virus (SFV) vector expression control with capsid expression enhancement. In this construct the lacZ is expressed as a C-lacZ fusion protein.

The plasmid contains the same silent mutations as pSP6-SFV4s in order to remove bacterial transcription promotors.

The capsid protein (C) of SFV contains a protease activity that cleaves the C protein off the growing polypeptide chain during biosynthesis. The C protease cleaves after the C-terminal W in the wild type sequence WSA. In recombinant proteins the C protease cleave many sequences equally well e.g. WSA, WGE, WMM, WSL and WAS. producing recombinant proteins with correct N-terminus. However, the sequence WDP, the consequence of using a BamH1 cloning site, is not cleaved.