Skip to main content
Addgene

pSFV-Helper1
(Plasmid #92073)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEM1
  • Backbone size w/o insert (bp) 2833
  • Total vector size (bp) 8194
  • Vector type
    Helper plasmid, for run-off transcription and RNA transfection of mammalian cells

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
  • Insert Size (bp)
    5361
  • GenBank ID

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Provides wild type structural Semliki forest virus proteins for packaging of recombinant RNA genomes into virus like particles.

Deposited for prof. Henrik Garoff.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFV-Helper1 was a gift from Henrik Garoff (Addgene plasmid # 92073 ; http://n2t.net/addgene:92073 ; RRID:Addgene_92073)

  • For your References section:

    A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Liljestrom P, Garoff H. Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed 1370252
Related items:
Commonly requested with: