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Purpose(Empty Backbone) Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSP6-SFV4
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Modifications to backboneDeletion AsuII to NdeI which removes the structural viral proteins.
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Vector typeEmpty expression plasmid, for run-off transcription and RNA transfection of mammalian cells
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Deposited for prof. Henrik Garoff.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSFV3 was a gift from Henrik Garoff (Addgene plasmid # 92072 ; http://n2t.net/addgene:92072 ; RRID:Addgene_92072) -
For your References section:
A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Liljestrom P, Garoff H. Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed 1370252
Map uploaded by the depositor.
