pSP6-SFV4s
(Plasmid
#92071)
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PurposePlasmid for in vitro transcription of full length Semilki Forest virus (SFV) RNA genomes, based on pSP6-SFV4, with silent mutations to remove bacterial transcription promotors from original sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM1
- Backbone size w/o insert (bp) 2833
- Total vector size (bp) 14288
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Vector typePlasmid for run-off transcription and RNA transfection of mammalian cells
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecDNA of complete semliki forest virus genome (SFV4)
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Insert Size (bp)11455
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GenBank ID
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The original plasmid pSP6-SFV4 is highly unstable, when grown in bacteria. The reason is bacterial transcription/translation of the structural viral proteins, of which the E1 protein appears to intoxicate the bacteria. In pSP6-SFV4s we introduced several silent mutations (around positions 3177, 4000 and 8081) to remove three potential bacterial transcription promotors. These changes were sufficient to stabilize the plasmid, but have not been published. Mathilda Sjöberg and Henrik Garoff
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP6-SFV4s was a gift from Henrik Garoff (Addgene plasmid # 92071) -
For your References section:
In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release. Liljestrom P, Lusa S, Huylebroeck D, Garoff H. J Virol. 1991 Aug;65(8):4107-13. PubMed 2072446