OptoEphB2KD-mCherry
(Plasmid #92044)

• Purpose: Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry
• Depositing Lab: Ji Yu
• Publication: optoEphB plasmids (unpublished)

Backbone

• Vector backbone: pmCherryN1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPHB2
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Entrez Gene: EPHB2 (a.k.a. BDPLT22, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5)
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

OptoEphB sequence encodes a N-term myristoylation signal peptide, AA565-986 of EphB2, and the PHR domain of Cry2olig (E490G).

This clone contains the K->M mutation in the kinase domain of EphB2 resulting in inactivation of the kinase.