OptoEphB2-mCherry
(Plasmid
#92042)
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PurposeExpresses OptoEphB2 labeled with mCherry
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherryN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPHB2
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Entrez GeneEPHB2 (a.k.a. BDPLT22, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5)
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
OptoEphB sequence encodes a N-term myristoylation signal peptide, AA565-986 of EphB2, and the PHR domain of Cry2olig (E490G).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OptoEphB2-mCherry was a gift from Ji Yu (Addgene plasmid # 92042 ; http://n2t.net/addgene:92042 ; RRID:Addgene_92042)