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mEos3-caRac1
(Plasmid #92041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEX
  • Total vector size (bp) 6424
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAC1
  • Species
    H. sapiens (human)
  • Mutation
    Q61L (constitutively active)
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos3 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEos3-caRac1 was a gift from Ji Yu (Addgene plasmid # 92041 ; http://n2t.net/addgene:92041 ; RRID:Addgene_92041)

  • For your References section:

    Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling. Das S, Yin T, Yang Q, Zhang J, Wu YI, Yu J. Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):E267-76. doi: 10.1073/pnas.1409667112. Epub 2015 Jan 5. 10.1073/pnas.1409667112 PubMed 25561548
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