mEos3-dnRac1
(Plasmid #92040)

• Purpose: Expresses mEos3 fusion with dnRac1 (T17N).
• Depositing Lab: Ji Yu
• Publication: Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):E267-76. doi: 10.1073/pnas.1409667112. Epub 2015 Jan 5.

Backbone

• Vector backbone: pTriEX
• Total vector size (bp): 6424
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAC1
• Species: H. sapiens (human)
• Mutation: T17N (dominant negative)
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEos3 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: mEos-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry