mEos3-dnRac1
(Plasmid
#92040)
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PurposeExpresses mEos3 fusion with dnRac1 (T17N).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTriEX
- Total vector size (bp) 6424
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAC1
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SpeciesH. sapiens (human)
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MutationT17N (dominant negative)
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
- Promoter CMV
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Tag
/ Fusion Protein
- mEos3 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer mEos-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEos3-dnRac1 was a gift from Ji Yu (Addgene plasmid # 92040 ; http://n2t.net/addgene:92040 ; RRID:Addgene_92040) -
For your References section:
Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling. Das S, Yin T, Yang Q, Zhang J, Wu YI, Yu J. Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):E267-76. doi: 10.1073/pnas.1409667112. Epub 2015 Jan 5. 10.1073/pnas.1409667112 PubMed 25561548
