CIB-VP64 (B1016)
(Plasmid #92037)

• Purpose: Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)
• Depositing Lab: Chandra Tucker
• Publication: Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3965
• Total vector size (bp): 5198
• Modifications to backbone: no mCherry tag.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIB1-VP64
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1224
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Fusion of plant CIB1 with VP64 activation domain. Intact NLS. No mCherry tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Resource Information

• A portion of this plasmid was derived from a plasmid made by: VP64 was amplified from a plasmid from Charlie Gersbach (pcDNA-dCas9-VP64)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry