CIB-VP16 (B1014)
(Plasmid #92036)

• Purpose: Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.
• Depositing Lab: Chandra Tucker
• Publication: Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5462
• Total vector size (bp): 6779
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIB1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1044
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: T7
• Tag / Fusion Protein:
  • Fusion of plant CIB1 (intact NLS) with VP16 activation domain

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry