CRY-Gal∆DD (B1013)
(Plasmid #92035)

• Purpose: Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES
• Depositing Lab: Chandra Tucker
• Publication: Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.

Backbone

• Vector backbone: pmCherryC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5270
• Total vector size (bp): 7373
• Modifications to backbone: IRES element between end of mCherry and SacI sites
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2103
• Mutation: Fusion of plant CRY2 to Gal4 binding domain (AA1-65), downstream of mCherry-IRES element
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI, SacI (not destroyed)
• 3′ cloning site: Not1 between linker of CRY2 and Gal4 BD (not destroyed)
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry